March 9, 2023. Our work using droplet-based CRISPR screens (SPEAC-seq) to identify mechanisms of astrocyte-microglia cross-talk is published in Science. Another fantastic collaboration with Mike Wheeler, Hong-Gyun Lee, and the Quintana Lab.
March 6, 2023. Our microfluidic-free scRNA-seq method (PIP-seq) is published in Nature Biotechnology. Updated BioRxiv pre-print is here. A great collaboration with Fluent Biosciences – kits are available if you’re interested in trying. ICC disclosures related to this work are found here.
Jan 4, 2023. Our paper, in collaboration with the Abate and Boritz Labs, is published in Nature. We used FIND-seq to sort and transcriptome sequence HIV DNA+ cells. Infected CD4 T cells have unique signatures that help to explain HIV persistence in vivo. Thanks to Nicolas Chomont for his excellent News & Views summary of our work (and work from the the Lichterfeld Lab published in the same issue).
Jan 4, 2023. Our droplet microfluidic method (FIND-seq) is published in Nature. In collaboration with the Quintana and Abate Labs, we sorted rare astrocyte subsets and discovered that NR3C2 and its corepressor NCOR2 limit XBP1-driven pathogenic astrocyte responses.
Nov 7, 2022. Sakshi passed her qualifying exam in Bioengineering!
Aug 7, 2022. Press for our work on HIV latency presented by Eli Boritz at the International Aids Conference. Briefly discussed by Dr. Fauci (skip to 7:45)!
June 14, 2022. New Preprint! We present a rapid and highly scalable single-cell RNA sequencing method that eliminates the need for microfluidics.
June, 2022. Kevin passed his qualifying exam in Bioengineering!
June, 2021. Iain receives an NIH New Innovator award to study CNS immune cell interactions using RABID-seq.
May, 2021. Sakshi and Kevin join the lab!
April 22, 2021. Rabies barcode interaction detection followed by sequencing (RABID-seq) is published in Science.
April 15, 2021. Iain receives a NIH NIAID K22 transition award to continue work on PCR-activated cell sorting of latent HIV.
Feb, 2021. Our paper describing a hybrid droplet-valve microfluidic device for assembling rationally reduced DNA libraries is published in PNAS. In collaboration with Bruk Mensa and Bill DeGrado, we use this technique study domain coupling in the bacterial histidine kinase CpxA.
Jan, 2021. The lab opens. Many thanks to the tremendous mentors and collaborators who made this journey possible. Special thanks to Adam Abate, Francisco Quintana and Eli Boritz!
Nov, 2020. Our paper describing a method for targeted barcoding without sorting is published in Analytical Chemistry.
Feb, 2020. Mike Wheeler’s scRNA-seq paper deconstructing astrocyte heterogeneity during experimental autoimmunity is published in Nature.