Infection with Human Immunodeficiency Virus (HIV) is incurable, despite drugs that effectively target the viral life cycle. The reservoir of cells containing replication-competent HIV that persists during drug therapy is poorly understood because these cells cannot be isolated to high purity. A major focus of the lab is isolating and sequencing latently infected CD4 T cells from people on long term effective anti-retorviral therapy.
Current projects focus on:
- Development of PCR activated cell sorting (PACS) for single cell analysis
- Application of PACS to isolate and RNA-sequence rare HIV-infected T cells from people on ART
- Extension of PACS to study protein and epigenetic signatures of HIV-infected T cells